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Proceedings Article

Comparison of resolution in tomographic diffractive microscopy using combinations of sample rotation and illumination rotation

[+] Author Affiliations
Stanislas Vertu, Jens Flügge

Physikalisch-Technische Bundesanstalt (Germany)

Etsuo Maeda, Jean-Jacques Delaunay

The Univ. of Tokyo (Japan)

Olivier Haeberlé

Univ. of Haute Alsace (France)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79041O (February 22, 2011); doi:10.1117/12.874446
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

Tomographic Diffractive Microscopy (TDM) is a technique, which permits to image transparent living specimens without staining. For weakly diffractive samples, the three-dimensional distribution of the complex Refractive Index (RI) can be reconstructed from the knowledge of the measured scattered fields sampled under various viewing and illumination angles, according to the diffraction tomography theorem. TDM is commonly implemented in two ways, by either rotating the sample illumination keeping the specimen fixed, or by rotating the sample using fixed illumination. Both methods present limitations. Under the first-order Born approximation, the varying illumination direction method presents a strong anisotropic resolution along the optical axis due to the so-called "missing cone" of non captured frequencies. The sample rotation method presents a better isotropic resolution, but with a reduced extension of the captured frequencies. In view of overcoming the limitations of each method, we have studied various techniques for expanding the Optical Transfer Function with a tomographic microscope by combining different configurations of the sample rotation method with the varying illumination direction method, in order to obtain a high and isotropic resolution. Using simulations, we investigate the performances of the different configurations we propose.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Stanislas Vertu ; Etsuo Maeda ; Jens Flügge ; Jean-Jacques Delaunay and Olivier Haeberlé
"Comparison of resolution in tomographic diffractive microscopy using combinations of sample rotation and illumination rotation", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79041O (February 22, 2011); doi:10.1117/12.874446; http://dx.doi.org/10.1117/12.874446


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