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Proceedings Article

Lens-less holographic microscope with high resolving power and no-distortion

[+] Author Affiliations
Kunihiro Sato, Osamu Murata

Univ. of Hyogo (Japan)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790402 (February 10, 2011); doi:10.1117/12.876563
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

A new method is developed for recording and reconstructing a microscopic high-resolution 3-D image with no distortion. Any imaging lens is not located between an object and a CCD in our optical system. An off-axis hologram with a large numerical aperture is recorded at once, and a complex-amplitude in-line hologram is extracted from the recorded off-axis hologram by applying the one-shot digital holography. A small complex-amplitude in-line hologram is generated for the reconstruction of microscopic high-resolution images by dividing the large hologram into a number of small holograms and by superimposing them. A focus-free image with high resolving power and no distortion is reconstructed from the generated complex-amplitude in-line hologram. Resolution higher than 1μm is obtained in the optical experiment, which can be improved up to the wave length. Microscopic high-resolution images with no distortion can also be observed for objects immersed in the liquid or in the solid by using the lens-less holographic microscope.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Kunihiro Sato and Osamu Murata
"Lens-less holographic microscope with high resolving power and no-distortion", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790402 (February 10, 2011); doi:10.1117/12.876563; http://dx.doi.org/10.1117/12.876563


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