Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Detection of pathogenic DNA at the single-molecule level

[+] Author Affiliations
Idir Yahiatène, Mark Schüttpelz

Univ. of Bielefeld (Germany)

Tobias Klamp, Markus Sauer

Julius-Maximilians-Univ. Würzburg (Germany)

Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050M (February 22, 2011); doi:10.1117/12.873850
Text Size: A A A
From Conference Volume 7905

  • Single Molecule Spectroscopy and Imaging IV
  • Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann
  • San Francisco, California, USA | January 22, 2011

abstract

We demonstrate ultrasensitive detection of pathogenic DNA in a homogeneous assay at the single-molecule level applying two-color coincidence analysis. The target molecule we quantify is a 100 nucleotide long synthetic single-stranded oligonucleotide adapted from Streptococcus pneumoniae , a bacterium causing lower respiratory tract infections. Using spontaneous hybridization of two differently fluorescing Molecular Beacons we demonstrate a detection sensitivity of 100 fM (10-13M) in 30 seconds applying a simple microfluidic device with a 100 μm channel and confocal two-color fluorescence microscopy.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Idir Yahiatène ; Tobias Klamp ; Mark Schüttpelz and Markus Sauer
"Detection of pathogenic DNA at the single-molecule level", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050M (February 22, 2011); doi:10.1117/12.873850; http://dx.doi.org/10.1117/12.873850


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.