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Proceedings Article

Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

[+] Author Affiliations
Ryan A. Colyer, Giuseppe Scalia, Shimon Weiss, Xavier Michalet

Univ. of California, Los Angeles (USA)

Federica A. Villa, Fabrizio Guerrieri, Franco Zappa, Sergio Cova

Politecnico di Milano (Italy)

Simone Tisa

Micro Photon Devices (Italy)

Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 790503 (February 28, 2011); doi:10.1117/12.874238
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From Conference Volume 7905

  • Single Molecule Spectroscopy and Imaging IV
  • Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann
  • San Francisco, California, USA | January 22, 2011

abstract

Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness, conformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with good statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition speed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in throughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS 1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Ryan A. Colyer ; Giuseppe Scalia ; Federica A. Villa ; Fabrizio Guerrieri ; Simone Tisa, et al.
"Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 790503 (February 28, 2011); doi:10.1117/12.874238; http://dx.doi.org/10.1117/12.874238


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