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Proceedings Article

Optical assessment of pathology in surgically resected tissues

[+] Author Affiliations
Ashley Laughney, Venkataramanan Krishnaswamy, Keith D. Paulsen, Brian W. Pogue

Dartmouth College (USA)

Wendy A. Wells

Dartmouth Hitchcock Medical Ctr. (USA)

Olga M. Conde

Univ. de Cantabria (Spain)

Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070P (February 11, 2011); doi:10.1117/12.874059
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From Conference Volume 7907

  • Biomedical Applications of Light Scattering V
  • Adam P. Wax; Vadim Backman
  • San Francisco, California, USA | January 22, 2011

abstract

Multi-spectral spatially modulated light is used to guide localized spectroscopy of surgically resected tissues for cancer involvement. Modulated imaging rapidly quantifies near-infrared optical parameters with sub-millimeter resolution over the entire field for identification of residual disease in resected tissues. Suspicious lesions are further evaluated using a spectroscopy platform designed to image thick tissue samples at a spatial resolution sensitive to the diagnostic gold standard, pathology. MI employs a spatial frequency domain sampling and model-based analysis of the spatial modulation transfer function to interpret a tissue's absorption and scattering parameters at depth. The spectroscopy platform employs a scanning-beam, telecentric dark-field illumination and confocal detection to image fields up to 1cm2 with a broadband source (480:750nm). The sampling spot size (100μm lateral resolution) confines the volume of tissue probed to within a few transport pathlengths so that multiple-scattering effects are minimized and simple empirical models may be used to analyze spectra. Localized spectroscopy of Intralipid and hemoglobin phantoms demonstrate insensitivity of recovered scattering parameters to changes in absorption, but a non-linear dependence of scattering power on Intralipid concentration is observed due to the phase sensitivity of the measurement system. Both systems were validated independently in phantom and murine studies. Ongoing work focuses on assessing the combined utility of these systems to identify cancer involvement in vitro, particularly in the margins of resected breast tumors.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Ashley Laughney ; Venkataramanan Krishnaswamy ; Wendy A. Wells ; Olga M. Conde ; Keith D. Paulsen, et al.
"Optical assessment of pathology in surgically resected tissues", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070P (February 11, 2011); doi:10.1117/12.874059; http://dx.doi.org/10.1117/12.874059


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