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Proceedings Article

Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermis studied by reflectance confocal microscopy

[+] Author Affiliations
S. L. Jacques, R. Samatham, K. G. Phillips

Oregon Health & Science Univ. (USA)

Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790706 (February 11, 2011); doi:10.1117/12.874060
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From Conference Volume 7907

  • Biomedical Applications of Light Scattering V
  • Adam P. Wax; Vadim Backman
  • San Francisco, California, USA | January 22, 2011

abstract

Optical clearing of mouse dermis by glycerol was tested by reflectance-mode confocal microscopy (rCSLM) using 488- nm light. The reflectance signal R(z) was acquired as a function of the depth of the focus (z) within the upper 100 μm of freshly excised mouse dermis. The results specify the scattering coefficient (μs [cm-1]) and the anisotropy of scattering (g [dimensionless]). The absorption is too low to exert an effect. The results, published in Samatham et al., Journal of Innovative Optical Health Sciences 2010, 3(3):183-188, described how the clearing effect of glycerol was to increase g toward nearly 1.0, while having only a modest effect on μs. In other words, glycerol caused light scattering to become very forward-directed, but did not strongly alter the number of scattering events per unit length of photon path. This paper discusses the possible mechanism of action that is responsible for this clearing effect.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

S. L. Jacques ; R. Samatham and K. G. Phillips
"Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermis studied by reflectance confocal microscopy", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790706 (February 11, 2011); doi:10.1117/12.874060; http://dx.doi.org/10.1117/12.874060


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