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Proceedings Article

Toward an on-chip multiplexed nucleic acid hybridization assay using immobilized quantum dot-oligonucleotide conjugates and fluorescence resonance energy transfer

[+] Author Affiliations
Anthony J. Tavares, M. Omair Noor, W. Russ Algar, Charles H. Vannoy, Lu Chen, Ulrich J. Krull

Univ. of Toronto Mississauga (Canada)

Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090X (February 11, 2011); doi:10.1117/12.877326
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From Conference Volume 7909

  • Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI
  • Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski
  • San Francisco, California, USA | January 22, 2011

abstract

Semiconductor quantum dots (QD) are a class of NP with photophysical properties that are ideally suited for optical multiplexing and use as donors in fluorescence resonance energy transfer (FRET). A new strategy is presented for the development of multiplexed DNA hybridization assays using immobilized QDs in a microfluidic system. Green- or red-emitting QDs were immobilized via self-assembly with a multidentate-thiol-derivatized glass slide, and subsequently conjugated with amine-terminated probe oligonucleotides using carbodiimide activation. Immobilized QD-probe conjugates were then passivated with adsorbed non-complementary oligonucleotides to achieve selectivity in microfluidic assays. Target nucleic acid sequences hybridized with QD-probe conjugates and were labeled with Cy3 or Alexa Fluor 647 as acceptor dyes for the QD donors, where FRET-sensitized dye emission provided a signal for the detection of picomolar quantities of target. The simultaneous immobilization of green- and red-emitting QDs at different ratios within a microfluidic channel was demonstrated as a step toward multiplexed assays.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Anthony J. Tavares ; M. Omair Noor ; W. Russ Algar ; Charles H. Vannoy ; Lu Chen, et al.
"Toward an on-chip multiplexed nucleic acid hybridization assay using immobilized quantum dot-oligonucleotide conjugates and fluorescence resonance energy transfer", Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090X (February 11, 2011); doi:10.1117/12.877326; http://dx.doi.org/10.1117/12.877326


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