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Proceedings Article

Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression

[+] Author Affiliations
Jianpeng Xue, Lingling Shan, Zhenzhen Tu, Hongyan Zhu, Haiyan Chen, Dawei Deng, Yueqing Gu

China Pharmaceutical Univ. (China)

Zhiyu Qian

Nanjing Univ. of Aeronautics and Astronautics (China)

Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100I (February 11, 2011); doi:10.1117/12.874014
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From Conference Volume 7910

  • Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III
  • Samuel Achilefu; Ramesh Raghavachari
  • San Francisco, California, USA | January 22, 2011

abstract

Signal transducer and activator of transcription (STAT) 5b is an important protein in JAK-STAT signal pathway and is responsible for the metastasis and proliferation of tumor cells. The determination of the STAT5b expression provides a way to study the mechanism of tumor progress. In this study, gold nanoparticles with different diameters were conjugated to the fluorescein modified STAT5b specific DNA sequence to form the beacon. The procedures for the beacon with better fluorescence properties were optimized. The fluorescence quenching and the recovery properties after hybridizing with mRNA of STAT5b were intensively investigated. Results indicate the gold nanoparticle based beacon is an effective probe for the determination of STAT5b protein expression in JAK-STAT signal pathway and has great potential in the study of drug screening and discovery.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jianpeng Xue ; Lingling Shan ; Zhenzhen Tu ; Hongyan Zhu ; Haiyan Chen, et al.
"Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100I (February 11, 2011); doi:10.1117/12.874014; http://dx.doi.org/10.1117/12.874014


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