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Proceedings Article

Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine

[+] Author Affiliations
Trisha Eustaquio, Christy L. Cooper, James F. Leary

Purdue Univ. (USA)

Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100O (February 11, 2011); doi:10.1117/12.874290
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From Conference Volume 7910

  • Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III
  • Samuel Achilefu; Ramesh Raghavachari
  • San Francisco, California, USA | January 22, 2011

abstract

In nanomedicine, biodistribution studies are critical to evaluate the safety and efficacy of nanoparticles. Currently, extensive biodistribution studies are hampered by the limitations of bulk tissue and single-cell imaging techniques. To ameliorate these limitations, we have developed a novel method for single nanoparticle detection that incorporates a conjugated oligonucleotide as a "nanobarcode" for detection via in situ PCR. This strategy magnifies the detection signal from single nanoparticles, facilitating rapid evaluation of nanoparticle uptake by cell type over larger areas. The nanobarcoding method can enable precise analysis of nanoparticle biodistributions and expedite translation of these nanoparticles to the clinic.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Trisha Eustaquio ; Christy L. Cooper and James F. Leary
"Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100O (February 11, 2011); doi:10.1117/12.874290; http://dx.doi.org/10.1117/12.874290


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