An investigation of the visible light glucose sensor by utilized nanoporous (NPS) silicon material as a sensitive layer was proposed. In the experiments, all studied NPS films are prepared by electrochemical anodization technique and the obtained depth are about 3.3 μm. The peak of PL-intensity curve is 610 nm under normal air condition. Based on its high surface to volume ratio (SVR) and unique light emission properties, the studied NPS glucose sensor has a high sensitivity and stability. The visible light response on the film surface switches obviously between distinct colors with/without glucose treatment. As compared with conventional electronic glucose sensor, the highly sensitive and obvious light switching characteristics would be important to related detection. It has a potential application on small-size, low-cost, portable and electric-free sensor systems. After the immersion treatment of 1M glucose solution on the studied NPS samples, an obvious blue-shift behavior of PL-feature (from 610 to 560 nm) is observed at room temperature. The color switching can be read by a naked-eye easily.© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.