Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Surface figure control for optical filters in fluorescence microscopy

[+] Author Affiliations
Yi Cao, Shuaifeng Zhao, Yongxi Zhang, Jing Ma, Xiaojun Yin

HB Optical Technology Co., Ltd. (China)

Lin Tang

Alantek International (USA)

Shuguo Fei

Shenyang Academy of Instrumentation Science (China)

Proc. SPIE 7995, Seventh International Conference on Thin Film Physics and Applications, 79951Z (February 17, 2011); doi:10.1117/12.888215
Text Size: A A A
From Conference Volume 7995

  • Seventh International Conference on Thin Film Physics and Applications
  • Junhao Chu; Zhanshan Wang
  • Shanghai, China | September 24, 2010

abstract

The request of fluorescence microscopy filters was introduced. Optimized filter construction which requires more than 100 layers stack on both sides was redesigned. Surface deformation of filters caused by stress of multi-layers film was studied. The stress deformation effected by different film material and thickness was analyzed. A new flatten surface method was used to improve the surface flatness to 0.25λ. Filter sets were manufactured and photomicrograph was obtained with high contrast and no deformations when these filter sets were applied in fluorescence microscopy.

© (2010) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Yi Cao ; Lin Tang ; Shuaifeng Zhao ; Yongxi Zhang ; Jing Ma, et al.
"Surface figure control for optical filters in fluorescence microscopy", Proc. SPIE 7995, Seventh International Conference on Thin Film Physics and Applications, 79951Z (February 17, 2011); doi:10.1117/12.888215; http://dx.doi.org/10.1117/12.888215


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.