Novel utilization of the outer membrane proteins for the identification and differentiation of pathogenic versus nonpathogenic microbial strains using mass spectrometry-based proteomics approach

Rabih E. Jabbour; Mary Wade; Samir V. Deshpande; Patrick McCubbin; A. Peter Snyder; Vicky Bevilacqua

doi:10.1117/12.921254

4 May 2012 Novel utilization of the outer membrane proteins for the identification and differentiation of pathogenic versus nonpathogenic microbial strains using mass spectrometry-based proteomics approach

Rabih E. Jabbour, Mary Wade, Samir V. Deshpande, Patrick McCubbin, A. Peter Snyder, Vicky Bevilacqua

Author Affiliations +

Proceedings Volume 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII; 835809 (2012) https://doi.org/10.1117/12.921254
Event: SPIE Defense, Security, and Sensing, 2012, Baltimore, Maryland, United States

Abstract

Mass spectrometry based proteomic approaches are showing promising capabilities in addressing various biological and biochemical issues. Outer membrane proteins (OMPs) are often associated with virulence in gram-negative pathogens and could prove to be excellent model biomarkers for strain level differentiation among bacteria. Whole cells and OMP extracts were isolated from pathogenic and non-pathogenic strains of Francisella tularensis, Burkholderia thailandensis, and Burkholderia mallei. OMP extracts were compared for their ability to differentiate and delineate the correct database organism to an experimental sample and for the degree of dissimilarity to the nearest-neighbor database strains. This study addresses the comparative experimental proteome analyses of OMPs vs. whole cell lysates on the strain-level discrimination among gram negative pathogenic and non-pathogenic strains.

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Rabih E. Jabbour, Mary Wade, Samir V. Deshpande, Patrick McCubbin, A. Peter Snyder, and Vicky Bevilacqua "Novel utilization of the outer membrane proteins for the identification and differentiation of pathogenic versus nonpathogenic microbial strains using mass spectrometry-based proteomics approach", Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 835809 (4 May 2012); https://doi.org/10.1117/12.921254

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KEYWORDS

Proteins

Pathogens

Databases

Organisms

Binary data

Bacteria

Mass spectrometry

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