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Proceedings Article

Electrical detection of specific versus non-specific binding events in breast cancer cells

[+] Author Affiliations
Benjamin C. King, Michael Clark, Thomas Burkhead, Palaniappan Sethu, Balaji Panchapakesan

Univ. of Louisville (United States)

Shesh Rai, Goetz Kloecker

James Graham Brown Cancer Ctr. (United States)

Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600S (October 10, 2012); doi:10.1117/12.929875
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From Conference Volume 8460

  • Biosensing and Nanomedicine V
  • Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi
  • San Diego, California, USA | August 12, 2012

abstract

Detection of circulating tumor cells (CTCs) from patient blood samples offers a desirable alternative to invasive tissue biopsies for screening of malignant carcinomas. A rigorous CTC detection method must identify CTCs from millions of other formed elements in blood and distinguish them from healthy tissue cells also present in the blood. CTCs are known to overexpress surface receptors, many of which aid them in invading other tissue, and these provide an avenue for their detection. We have developed carbon nanotube (CNT) thin film devices to specifically detect these receptors in intact cells. The CNT sidewalls are functionalized with antibodies specific to Epithelial Cell Adhesion Molecule (EpCAM), a marker overexpressed by breast and other carcinomas. Specific binding of EpCAM to anti-EpCAM antibodies causes a change in the local charge environment of the CNT surface which produces a characteristic electrical signal. Two cell lines were tested in the device: MCF7, a mammary adenocarcinoma line which overexpresses EpCAM, and MCF10A, a non-tumorigenic mammary epithelial line which does not. Introduction of MCF7s caused significant changes in the electrical conductance of the devices due to specific binding and associated charge environment change near the CNT sidewalls. Introduction of MCF10A displays a different profile due to purely nonspecific interactions. The profile of specific vs. nonspecific interaction signatures using carbon based devices will guide development of this diagnostic tool towards clinical sample volumes with wide variety of markers. © (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Benjamin C. King ; Michael Clark ; Thomas Burkhead ; Palaniappan Sethu ; Shesh Rai, et al.
" Electrical detection of specific versus non-specific binding events in breast cancer cells ", Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600S (October 10, 2012); doi:10.1117/12.929875; http://dx.doi.org/10.1117/12.929875


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