Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Concentration methods for high-resolution THz spectroscopy of nucleic-acid biomolecules and crystals

[+] Author Affiliations
E. R. Brown

Wright State Univ. (United States)

W. Zhang

North Carolina State Univ. (United States)

E. A. Mendoza

Redondo Optics, Inc. (United States)

Y. Kuznetsova, S. R. J. Brueck

The Univ. of New Mexico (United States)

M. Rahman, M. L. Norton

Marshall Univ. (United States)

Proc. SPIE 8221, Optical Interactions with Tissue and Cells XXIII, 82210S (February 8, 2012); doi:10.1117/12.912219
Text Size: A A A
From Conference Volume 8221

  • Optical Interactions with Tissue and Cells XXIII
  • E. Duco Jansen; Robert J. Thomas
  • San Francisco, California, USA | January 21, 2012

abstract

Biomolecules can exhibit low-lying vibrational modes in the THz region which are detectable in transmission given a strong molecular dipole moment and optical depth, and a spectrometer of adequate sensitivity. The nucleic acids are particularly interesting because of applications such as label-free gene assay, bio-agent detection, etc. However for nucleic acids, sample preparation and THz coupling are of paramount importance because of the strong absorption by liquid water and the small concentration of molecules present in physiological solutions. Concentration methods become necessary to make the THz vibrational modes detectable, either by concentrating the nucleic-acid sample itself in a small volume but large area, or by concentrating the THz radiation down to the volume of the sample. This paper summarizes one type of the first method: nanofluidic channel arrays for biological nucleic acids; and two types of the second method: (1) a circular-waveguide pinhole, and (2) a circular-waveguide, conical-horn coupling structure, both for DNA crystals. The first method has been demonstrated on a very short artificial nucleic acid [small-interfering (si) RNA (17-to-25 bp)] and a much longer, biological molecule [Lambda-phage DNA (48.5 kbp)]. The second method has been demonstrated on small (~100 micron) single crystals of DNA grown by the sitting-drop method.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

E. R. Brown ; W. Zhang ; E. A. Mendoza ; Y. Kuznetsova ; S. R. J. Brueck, et al.
"Concentration methods for high-resolution THz spectroscopy of nucleic-acid biomolecules and crystals", Proc. SPIE 8221, Optical Interactions with Tissue and Cells XXIII, 82210S (February 8, 2012); doi:10.1117/12.912219; http://dx.doi.org/10.1117/12.912219


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

 

 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.