0

Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods

[+] Author Affiliations
Hyoungseon Choi, Kwang Bok Kim, Changsu Jun, Taek Dong Chung, Hee Chan Kim

Seoul National Univ. (Korea, Republic of)

Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85484A (October 24, 2012); doi:10.1117/12.1000245
Text Size: A A A
From Conference Volume 8548

  • Nanosystems in Engineering and Medicine
  • Sang H. Choi; Jin-Ho Choy; Uhn Lee; Vijay K. Varadan
  • Incheon, Republic of Korea | September 10, 2012

abstract

In clinical and biological fields, circulating tumor cells (CTCs) attracts much attention for the valuable information about cancer progression, cancer status, and prognosis after the treatment with metastatic cancer. Recently, many researchers have studied to count CTCs efficiently. Representative methods of CTC detection are the immune-reaction based method and the morphology-based method. However, the immune-reaction based method is weak due to the imperfect markers, and morphology-based method has a defect because of the unclear criterion. In this paper, we described the CTC detection system based on flow cytometry technique with morphology and immune reaction based methods. The size and the immune-reaction information can be simultaneously obtained from DC impedance based detection and fluorescence detection, respectively. The performance of our system was evaluated with fluorescence beads. To apply the proposed system to biological samples, the human ovarian cancer cell lines (OVCAR-3) suspended in phosphate buffered saline (PBS) were tested. OVCAR-3 cells were stained by fluorescence tagged anti-epithelial cancer adhesion molecule (EpCAM). The portable flow cytometer system could detect the cancer cells with these methods. The proposed system has sufficient potential for point-of-care testing type cancer cell counter and many valuable clinical applications in the near future. © (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Hyoungseon Choi ; Kwang Bok Kim ; Changsu Jun ; Taek Dong Chung and Hee Chan Kim
" A portable microfluidic chip system for cancer diagnosis with simultaneous detection methods ", Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85484A (October 24, 2012); doi:10.1117/12.1000245; http://dx.doi.org/10.1117/12.1000245


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
 

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In