Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Wide field-of-view microscopy with Talbot pattern illumination

[+] Author Affiliations
Jigang Wu, Guangshuo Liu

Shanghai Jiao Tong Univ. (China)

Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85530B (December 11, 2012); doi:10.1117/12.999603
Text Size: A A A
From Conference Volume 8553

  • Optics in Health Care and Biomedical Optics V
  • Qingming Luo; Ying Gu; Xingde D. Li
  • Beijing, China | November 05, 2012

abstract

Wide field-of-view (FOV) microscopy is useful for high-throughput applications because of the capability to obtain large amount of information from a single image. One way to implement a wide FOV microscope is to scan the sample with a two-dimensional focus grid. The transmission or reflection of the focal spots can then be used to reconstruct the sample image. This scheme is effectively a parallel scanning optical microscope (SOM), where the FOV depends on the area of the focus grid and the imaging resolution depends on the spot size of the foci. We use the Talbot image of a twodimensional aperture grid as the focus grid and developed a wide FOV microscope. Preliminary experimental results show the capability of our microscope to acquire wide FOV images of US air force target and MCF-7 cancer cell samples. Fluorescence images of fluorescence beads are also acquired. Because the diffraction of incident beam by the aperture grid contains complicated angular frequencies, the focal spots in Talbot pattern cannot be approximated as Gaussian beams as in conventional SOM. We characterized the focal spots in Talbot pattern and studied the evolution of the full width at half maximum (FWHM). We also simulated the SOM imaging under Talbot pattern illumination using the razor blade as the sample objects.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jigang Wu and Guangshuo Liu
"Wide field-of-view microscopy with Talbot pattern illumination", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85530B (December 11, 2012); doi:10.1117/12.999603; http://dx.doi.org/10.1117/12.999603


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.