Accurate and in vivo characterization of structural, functional, and molecular characteristics of biological tissue will
facilitate quantitative diagnosis, therapeutic guidance, and outcome assessment in many clinical applications, such as
wound healing, cancer surgery, and organ transplantation. However, many clinical imaging systems have limitations and
fail to provide noninvasive, real time, and quantitative assessment of biological tissue in an operation room. To
overcome these limitations, we developed and tested a multiview hyperspectral imaging system. The multiview
hyperspectral imaging system integrated the multiview and the hyperspectral imaging techniques in a single portable
unit. Four plane mirrors are cohered together as a multiview reflective mirror set with a rectangular cross section. The
multiview reflective mirror set was placed between a hyperspectral camera and the measured biological tissue. For a
single image acquisition task, a hyperspectral data cube with five views was obtained. The five-view hyperspectral image
consisted of a main objective image and four reflective images. Three-dimensional topography of the scene was achieved
by correlating the matching pixels between the objective image and the reflective images. Three-dimensional mapping of
tissue oxygenation was achieved using a hyperspectral oxygenation algorithm. The multiview hyperspectral imaging
technique is currently under quantitative validation in a wound model, a tissue-simulating blood phantom, and an in vivo
biological tissue model. The preliminary results have demonstrated the technical feasibility of using multiview
hyperspectral imaging for three-dimensional topography of tissue functional properties.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation
Shiwu Zhang ; Peng Liu ; Jiwei Huang and Ronald Xu
"Multiview hyperspectral topography of tissue structural and functional characteristics", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85530P (December 11, 2012); doi:10.1117/12.1000006; http://dx.doi.org/10.1117/12.1000006
