0

Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Studying the role of macrophages in circulating prostate cancer cells by in vivo flow cytometry

[+] Author Affiliations
Xiaojun Cui, Jin Guo, Xunbin Wei

Fudan Univ. (China)

Zhengqin Gu

Shanghai Jiao Tong Univ. (China)

Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85530W (December 11, 2012); doi:10.1117/12.2001640
Text Size: A A A
From Conference Volume 8553

  • Optics in Health Care and Biomedical Optics V
  • Qingming Luo; Ying Gu; Xingde D. Li
  • Beijing, China | November 05, 2012

abstract

Metastasis is a very complicated multi-step process and accounts for the low survival rate of the cancerous patients. To metastasize, the malignant cells must detach from the primary tumor and migrate to secondary sites in the body through either blood or lymph circulation. Macrophages appear to be directly involved in tumor progression and metastasis. However, the role of macrophages in affecting cancer metastasis has not been fully elucidated. Here, we have utilized an emerging technique, namely in vivo flow cytometry (IVFC) to study the depletion kinetics of circulating prostate cancer cells in mice and how depletion of macrophages by the liposome-encapsulated clodronate affects the depletion kinetics. Our results show different depletion kinetics of PC-3 cells between macrophage-deficient group and the control group. The number of circulating tumor cells (CTCs) in macrophage-deficient group decreases in a slower manner compared to the control mice group. The differences in depletion kinetics indicate that the absence of macrophages facilitates the stay of prostate cancer cells in circulation. We speculate that macrophages might be able to arrest, phagocytose and digest PC-3 cells. Therefore, the phagocytosis may mainly contribute to the depletion kinetic differences. The developed methods here would be useful to study the relationship between macrophages and tumor metastasis in small animal cancer model.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Xiaojun Cui ; Jin Guo ; Zhengqin Gu and Xunbin Wei
"Studying the role of macrophages in circulating prostate cancer cells by in vivo flow cytometry", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85530W (December 11, 2012); doi:10.1117/12.2001640; http://dx.doi.org/10.1117/12.2001640


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
 

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In