Characterization and differentiation of normal and abnormal semen samples using micro-Raman spectroscopy

Zufang Huang; Xiwen Chen; Jinhua Chen; Yongzeng Li; Jinping Lei; Rong Chen

doi:10.1117/12.1000057

11 December 2012 Characterization and differentiation of normal and abnormal semen samples using micro-Raman spectroscopy

Zufang Huang, Xiwen Chen, Jinhua Chen, Yongzeng Li, Jinping Lei, Rong Chen

Author Affiliations +

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 85531U (2012) https://doi.org/10.1117/12.1000057
Event: Photonics Asia, 2012, Beijing, China

Abstract

Growth in the percentage of male infertility has caused extensive concerns. The fast and reliable method is urgently required for diagnosis of semen samples. In our study, micro-Raman spectroscopy was employed to characterize and differentiate the normal and abnormal semen samples based on the differences of their specific Raman spectra which originated from biochemical components. Our preliminary results demonstrate that micro-Raman spectroscopy combined with multivariate analysis methods has the potential of being used to detect and differentiate semen samples.

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Zufang Huang, Xiwen Chen, Jinhua Chen, Yongzeng Li, Jinping Lei, and Rong Chen "Characterization and differentiation of normal and abnormal semen samples using micro-Raman spectroscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531U (11 December 2012); https://doi.org/10.1117/12.1000057

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KEYWORDS

Raman spectroscopy

Micro raman spectroscopy

Statistical analysis

Luminescence

Plasma

FT-IR spectroscopy

Molecular spectroscopy

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