Brain cancer probed by native fluorescence and stokes shift spectroscopy

Yan Zhou; Cheng-hui Liu; Yong He; Yang Pu; Qingbo Li; Wei Wang; Robert R. Alfano

doi:10.1117/12.999463

11 December 2012 Brain cancer probed by native fluorescence and stokes shift spectroscopy

Yan Zhou, Cheng-hui Liu, Yong He, Yang Pu, Qingbo Li, Wei Wang, Robert R. Alfano

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 85531V (2012) https://doi.org/10.1117/12.999463
Event: Photonics Asia, 2012, Beijing, China

Abstract

Optical biopsy spectroscopy was applied to diagnosis human brain cancer in vitro. The spectra of native fluorescence, Stokes shift and excitation spectra were obtained from malignant meningioma, benign, normal meningeal tissues and acoustic neuroma benign tissues. The wide excitation wavelength ranges were used to establish the criterion for distinguishing brain diseases. The alteration of fluorescence spectra between normal and abnormal brain tissues were identified by the characteristic fluorophores under the excitation with UV to visible wavelength range. It was found that the ratios of the peak intensities and peak position in both spectra of fluorescence and Stokes shift may be used to diagnose human brain meninges diseases. The preliminary analysis of fluorescence spectral data from cancer and normal meningeal tissues by basic biochemical component analysis model (BBCA) and Bayes classification model based on statistical methods revealed the changes of components, and classified the difference between cancer and normal human brain meningeal tissues in a predictions accuracy rate is 0.93 in comparison with histopathology and immunohistochemistry reports (gold standard).

Citation Download Citation

Yan Zhou, Cheng-hui Liu, Yong He, Yang Pu, Qingbo Li, Wei Wang, and Robert R. Alfano "Brain cancer probed by native fluorescence and stokes shift spectroscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 85531V (11 December 2012); https://doi.org/10.1117/12.999463

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