The molecular characterization of ABO blood types, which is clinically significant in blood transfusion, has clinical and
anthropological importance. Polymerase chain reaction sequence-based typing (PCR-SBT) is one of the most commonly
used methods for the analysis of genetic bases of ABO blood types. However, such methods as PCR-SBT are
time-consuming and are high in demand of equipments and manipulative skill. Here we showed that membrane
electrophoresis based SERS method employed for studying the molecular bases of ABO blood types can provide rapidand
easy-operation with high sensitivity and specificity. The plasma proteins were firstly purified by membrane
electrophoresis and then mixed with silver nanoparticles to perform SERS detection. We use this method to classify
different blood types, including blood type A (n=13), blood type B (n=9) and blood type O (n=10). Combination of
principal component analysis (PCA) and liner discriminant analysis (LDA) was then performed on the SERS spectra of
purified albumin, showing good classification results among different blood types. Our experimental outcomes represent
a critical step towards the rapid, convenient and accurate identification of ABO blood types.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jing Wang ; Juqiang Lin ; Zufang Huang ; Liqing Sun ; Yonghong Shao, et al.
"Study of ABO blood types by combining membrane electrophoresis with surface-enhanced Raman spectroscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855323 (December 11, 2012); doi:10.1117/12.1000238; http://dx.doi.org/10.1117/12.1000238