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Proceedings Article

Study of ABO blood types by combining membrane electrophoresis with surface-enhanced Raman spectroscopy

[+] Author Affiliations
Jing Wang, Juqiang Lin, Zufang Huang, Peng Lu, Wei Shi, Jinyong Lin, Rong Chen

Fujian Normal Univ. (China)

Liqing Sun

First Hospital of Fuzhou (China)

Yonghong Shao

Shenzhen Univ. (China)

Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855323 (December 11, 2012); doi:10.1117/12.1000238
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From Conference Volume 8553

  • Optics in Health Care and Biomedical Optics V
  • Qingming Luo; Ying Gu; Xingde D. Li
  • Beijing, China | November 05, 2012

abstract

The molecular characterization of ABO blood types, which is clinically significant in blood transfusion, has clinical and anthropological importance. Polymerase chain reaction sequence-based typing (PCR-SBT) is one of the most commonly used methods for the analysis of genetic bases of ABO blood types. However, such methods as PCR-SBT are time-consuming and are high in demand of equipments and manipulative skill. Here we showed that membrane electrophoresis based SERS method employed for studying the molecular bases of ABO blood types can provide rapidand easy-operation with high sensitivity and specificity. The plasma proteins were firstly purified by membrane electrophoresis and then mixed with silver nanoparticles to perform SERS detection. We use this method to classify different blood types, including blood type A (n=13), blood type B (n=9) and blood type O (n=10). Combination of principal component analysis (PCA) and liner discriminant analysis (LDA) was then performed on the SERS spectra of purified albumin, showing good classification results among different blood types. Our experimental outcomes represent a critical step towards the rapid, convenient and accurate identification of ABO blood types.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jing Wang ; Juqiang Lin ; Zufang Huang ; Liqing Sun ; Yonghong Shao, et al.
"Study of ABO blood types by combining membrane electrophoresis with surface-enhanced Raman spectroscopy", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855323 (December 11, 2012); doi:10.1117/12.1000238; http://dx.doi.org/10.1117/12.1000238


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