Design of hybrid optical tweezers system for automated 3D micro manipulation

Yoshio Tanaka; Shogo Tsutsui; Hiroyuki Kitajima

doi:10.1117/12.980463

18 December 2012 Design of hybrid optical tweezers system for automated 3D micro manipulation

Yoshio Tanaka, Shogo Tsutsui, Hiroyuki Kitajima

Proceedings Volume 8550, Optical Systems Design 2012; 85501X (2012) https://doi.org/10.1117/12.980463
Event: SPIE Optical Systems Design, 2012, Barcelona, Spain

Abstract

In our previous paper, on dynamic handling of massive micro-bead arrays, we developed a hybrid optical tweezers system consisting of two multi-beam techniques: the GPC method and the galvano mirrors (GMs) scanning method. This system had high versatility for manipulating massive arrays, but arrays formed by its GM scanning tweezers could be handled only in a two-and-half dimensional (2.5D) working space. This limitation arose from the low bandwidth of the Z-axis manipulation due to the lens translation using a linear stage. For true 3D controlled manipulation of multiple micro-beads, in this paper, we redesign the GM scanning part of the previous hybrid system using an electrically focus-tunable lens with high bandwidth. The optical structure is linked to a commercially available microscope via its epifluorescence port. One set of optical tweezers based on the GPC uses the p-polarized beam, and the other set based on the GM scanning uses the s-polarized beam. In the results of the 3D manipulation experiment, the controlled rotation of five beads forming a pentagon and that of four beads forming a tetrahedron about arbitrary axes are demonstrated.

Citation Download Citation

Yoshio Tanaka, Shogo Tsutsui, and Hiroyuki Kitajima "Design of hybrid optical tweezers system for automated 3D micro manipulation", Proc. SPIE 8550, Optical Systems Design 2012, 85501X (18 December 2012); https://doi.org/10.1117/12.980463

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KEYWORDS

Optical tweezers

3D image processing

Optical design

Computing systems

Microscopes

Mirrors

Optical scanning systems

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