Particle position measuring with optical tweezers using video processing

Sławomir Drobczyński; Marcin Bacia; Marta Woźniak; Krzysztof Symonowicz

doi:10.1117/12.2007276

18 December 2012 Particle position measuring with optical tweezers using video processing

Sławomir Drobczyński, Marcin Bacia, Marta Woźniak, Krzysztof Symonowicz

Proceedings Volume 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 86970X (2012) https://doi.org/10.1117/12.2007276
Event: 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2012, Ostravice, Czech Republic

Abstract

The stiffness of a single optical trap is a basic parameter of optical manipulators and detecting the displacement of trapped object belongs to fundamental measurements [1]. The trap stiffness can be determined by position measurements of oscillating (with high frequency) dielectric microsphere. Usually the signal is captured by simple detector like quadrant photodiode [2]. However, this method is hard to apply when working in multitrap mode, especially in case of holographic optical tweezers. In multitrap mode, widely used method is video image processing focused on determining the center of mass of an object [3]. In this contribution we present the method for position measurment based on video sequence analysis from fast camera. The position is obtained by correlating the video frame with templates of known positions of that object. This algorithm can work on several objects, finding their positions independently. It has also scalable accuracy up to sub-pixel level.

Citation Download Citation

Sławomir Drobczyński, Marcin Bacia, Marta Woźniak, and Krzysztof Symonowicz "Particle position measuring with optical tweezers using video processing", Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970X (18 December 2012); https://doi.org/10.1117/12.2007276

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