The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on
microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify
different species. The investigations presented here include identification of different bacteria strains (biofilm-positive
and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our
investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant
and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not
form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation
Silvie Bernatová ; Ota Samek ; Zdeněk Pilát ; Mojmír Šerý ; Jan Ježek, et al.
"Raman spectroscopy for bacterial identification and characterization", Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970Y (December 18, 2012); doi:10.1117/12.2010539; http://dx.doi.org/10.1117/12.2010539
