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Proceedings Article

Raman spectroscopy for bacterial identification and characterization

[+] Author Affiliations
Silvie Bernatová, Ota Samek, Zdeněk Pilát, Mojmír Šerý, Jan Ježek, Vladislav Krzyžánek, Pavel Zemánek

Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)

Filip Růžička

Masaryk Univ. (Czech Republic)

Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970Y (December 18, 2012); doi:10.1117/12.2010539
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From Conference Volume 8697

  • 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics
  • Jan Peřina; Libor Nozka; Miroslav Hrabovský; Dagmar Senderáková; Waclaw Urbańczyk; Ondrej Haderka
  • Ostravice, Czech Republic | September 03, 2012

abstract

The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory - how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor. © (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Silvie Bernatová ; Ota Samek ; Zdeněk Pilát ; Mojmír Šerý ; Jan Ježek, et al.
" Raman spectroscopy for bacterial identification and characterization ", Proc. SPIE 8697, 18th Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 86970Y (December 18, 2012); doi:10.1117/12.2010539; http://dx.doi.org/10.1117/12.2010539


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