Photoinduced conformational changes of human serum albumin bound to protoporphyrin IX and hemin

Sarah C. Rozinek; Lorenzo Brancaleon

doi:10.1117/12.2004325

15 February 2013 Photoinduced conformational changes of human serum albumin bound to protoporphyrin IX and hemin

Sarah C. Rozinek, Lorenzo Brancaleon

Proceedings Volume 8579, Optical Interactions with Tissue and Cells XXIV; 85790G (2013) https://doi.org/10.1117/12.2004325
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Irradiation of porphyrins bound to proteins applies to photodynamic cancer therapy, photo-reduction of water, and the possibility of modifying proteins to impart new functions. Upon binding Protoporphyrin IX (PPIX) and hemin, respectively, to human albumin (HSA), the bound products’ response to low-dose irradiation at pH 7.4 is examined in this study. Spectroscopic data suggests that irradiation of PPIX when bound to HSA causes small secondary and tertiary protein conformational changes. Alternately, sizeable alterations are not seen when hemin bound to HSA is irradiated. This difference indicates a different photophysical mechanism for PPIX than for hemin.

Citation Download Citation

Sarah C. Rozinek and Lorenzo Brancaleon "Photoinduced conformational changes of human serum albumin bound to protoporphyrin IX and hemin", Proc. SPIE 8579, Optical Interactions with Tissue and Cells XXIV, 85790G (15 February 2013); https://doi.org/10.1117/12.2004325

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