The contribution of specific organelles to side scatter

Judith R. Mourant; Oana C. Marina; Claire K. Sanders

doi:10.1117/12.2008437

21 February 2013 The contribution of specific organelles to side scatter

Judith R. Mourant, Oana C. Marina, Claire K. Sanders

Proceedings Volume 8592, Biomedical Applications of Light Scattering VII; 85920L (2013) https://doi.org/10.1117/12.2008437
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Knowledge of which cellular structures scatter light is needed to fully utilize the information available from light scattering measurements of cells and tissues. To determine how specific organelles contribute to light scattering, wide angle side scattering was imaged simultaneously with fluorescence from specific organelles for thousands of cells using flow cytometry. Images were obtained with different depth of field conditions and analyzed with different assumptions. Both sets of data demonstrated that mitochondria and lysosomes, contribute similarly to side scatter. The nucleus contributes as much or more light scatter than either the mitochondria or the lysosomes.

Citation Download Citation

Judith R. Mourant, Oana C. Marina, and Claire K. Sanders "The contribution of specific organelles to side scatter", Proc. SPIE 8592, Biomedical Applications of Light Scattering VII, 85920L (21 February 2013); https://doi.org/10.1117/12.2008437

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available