0

Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Suppressing inflammation from inside out with novel NIR visible perfluorocarbon nanotheranostics

[+] Author Affiliations
Jelena M. Janjic, Sravan K. Patel, John A. Pollock, Erin DiVito, Michael Cascio

Duquesne Univ. (United States)

Michael J. Patrick

Carnegie Mellon Univ. (United States)

Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960L (February 21, 2013); doi:10.1117/12.2004625
Text Size: A A A
From Conference Volume 8596

  • Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
  • Samuel Achilefu; Ramesh Raghavachari
  • San Francisco, California, USA | February 02, 2013

abstract

Highly innovative multimodal perfluorocarbon (PFC) nanoemulsions are presented. They serve simultaneously as dual-mode imaging reagents (NIR and 19F MRI), and drug delivery vehicles for water insoluble cyclooxgenase-2 (COX-2) inhibitors. These features qualify them as theranostic. Cancer progression and metastasis are highly influenced by tumor microenvironment and inflammation. Infiltration of primary tumors with inflammation-promoting cells (e.g. macrophages) is a negative prognostic factor for cancer patient survival. We postulate that the suppression of COX-2 enzyme in macrophages by theranostic PFC nanoemulsions will result in changes in macrophage levels of accumulation in tumors and/or their phenotype, which can suppress tumor- promoting activity. The presented theranostic nanoemulsions are designed to label immune cells such as macrophages, and deliver celecoxib, a COX-2 inhibitor. The designed theranostic incorporates two fluorescent reporters: a near-infrared (NIR) fluorescent dye for improved optical in vivo imaging, and a distinct fluorescent dye for histological analysis of excised tissues. A high content of PFC in the theranostic allows 19F MRI to quantitatively assess the distribution of the injected nanomedicine in the peritumoral area, and measure tumor-associated inflammation, while 1H MRI provides anatomical context. NIR imaging is used as a complementary in vivo technique to assess biodistribution of the theranostic. We report preparation and characterization of the nanoemulsions’ colloidal and optical stability, in vitro toxicity, and imaging capabilities. This theranostic offers flexibility for in vitro and in vivo inflammation imaging and histological analysis using three different imaging functionalities (fluorescence, NIR and 19F MRI), advancing the monitoring and modulating of tumor-infiltrating immune cells in vivo. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jelena M. Janjic ; Sravan K. Patel ; Michael J. Patrick ; John A. Pollock ; Erin DiVito, et al.
" Suppressing inflammation from inside out with novel NIR visible perfluorocarbon nanotheranostics ", Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960L (February 21, 2013); doi:10.1117/12.2004625; http://dx.doi.org/10.1117/12.2004625


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
 

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In