Dynamic SERS imaging with gold nanoparticles transported in a living cell

Katsumasa Fujita; Jun Ando; Nicholas I. Smith; Satoshi Kawata

doi:10.1117/12.2005820

21 February 2013 Dynamic SERS imaging with gold nanoparticles transported in a living cell

Katsumasa Fujita, Jun Ando, Nicholas I. Smith, Satoshi Kawata

Proceedings Volume 8597, Plasmonics in Biology and Medicine X; 859707 (2013) https://doi.org/10.1117/12.2005820
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Surface enhanced Raman scattering (SERS) has been used to detect biological molecules at a low concentration. We developed a rapid Raman imaging system, which can image dynamic activity of SERS agents, such as gold nanoparticles, in a living cell and the temporal behaviors of SERS spectra. Combination of slit scanning and an EM-CCD camera for measuring SERS spectra enables us to obtain a SERS image in a few seconds. The system can also be used to track a single particle moving in a cell with a laser focus and measure SERS spectra with a temporal resolution of 50 msec. By using the developed microscope systems, we monitored the change of SERS spectra associated cell transportation functions.

Citation Download Citation

Katsumasa Fujita, Jun Ando, Nicholas I. Smith, and Satoshi Kawata "Dynamic SERS imaging with gold nanoparticles transported in a living cell", Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 859707 (21 February 2013); https://doi.org/10.1117/12.2005820

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