Raman microspectroscopy for visualization of peripheral nerves

Takeo Minamikawa; Yoshinori Harada; Noriaki Koizumi; Tetsuro Takamatsu

doi:10.1117/12.2001015

22 February 2013 Raman microspectroscopy for visualization of peripheral nerves

Takeo Minamikawa, Yoshinori Harada, Noriaki Koizumi, Tetsuro Takamatsu

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85880P (2013) https://doi.org/10.1117/12.2001015
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The peripheral nervous system plays an important role in motility, sensory, and autonomic functions of the human body. Preservation of peripheral nerves in surgery is essential for improving quality of life of patients. To preserve peripheral nerves, detection of ne peripheral nerves that cannot be identi ed by human eye or under white light imaging is necessary. In this study, we sought to provide a proof-of-principle demonstration of a label-free detection technique of peripheral nerve tissues against adjacent tissues that employs spontaneous Raman microspectroscopy. A line-illumination confocal Raman microscope was used for the experiment. A laser operating at the wavelength of 532 nm was used as an excitation laser light. We obtained Raman spectra of peripheral nerve, brous connective tissue, skeletal muscle, blood vessel, and adipose tissue of Wistar rats, and extracted speci c spectral features of peripheral nerves and adjacent tissues. By applying multivariate image analysis, peripheral nerves were clearly detected against adjacent tissues without any preprocessing neither xation nor staining. These results suggest the potential of the Raman spectroscopic observation for noninvasive and label-free nerve detection, and we expect this method could be a key technique for nerve-sparing surgery.

Citation Download Citation

Takeo Minamikawa, Yoshinori Harada, Noriaki Koizumi, and Tetsuro Takamatsu "Raman microspectroscopy for visualization of peripheral nerves", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880P (22 February 2013); https://doi.org/10.1117/12.2001015

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available