In vivo reactive neural plasticity investigation by means of correlative two photon: electron microscopy

A. L. Allegra Mascaro; P. Cesare; L. Sacconi; G. Grasselli; G. Mandolesi; B. Maco; G. Knott; L. Huang; V. De Paola; P. Strata; F. S. Pavone

doi:10.1117/12.2005953

22 February 2013 In vivo reactive neural plasticity investigation by means of correlative two photon: electron microscopy

A. L. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. Knott, L. Huang, V. De Paola, P. Strata, F. S. Pavone

Author Affiliations +

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881A (2013) https://doi.org/10.1117/12.2005953
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In the adult nervous system, different populations of neurons correspond to different regenerative behavior. Although previous works showed that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury¹, we have hitherto no details about the real dynamics of fiber regeneration. We set up a model of singularly axotomized climbing fibers (CF) to investigate their reparative properties in the adult central nervous system (CNS) in vivo. Time lapse two-photon imaging has been combined to laser nanosurgery^{2, 3} to define a temporal pattern of the degenerative event and to follow the structural rearrangement after injury. To characterize the damage and to elucidate the possible formation of new synaptic contacts on the sprouted branches of the lesioned CF, we combined two-photon in vivo imaging with block face scanning electron microscopy (FIB-SEM). Here we describe the approach followed to characterize the reactive plasticity after injury.

Citation Download Citation

A. L. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. Knott, L. Huang, V. De Paola, P. Strata, and F. S. Pavone "In vivo reactive neural plasticity investigation by means of correlative two photon: electron microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881A (22 February 2013); https://doi.org/10.1117/12.2005953

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available