Multimodal nonlinear optical microscopy used to discriminate human colon cancer

Javier Adur; Vitor B. Pelegati; Mariana Bianchi; André A. de Thomaz; Mariana O. Baratti; Hernandes F. Carvalho; Víctor H. Casco; Carlos L. Cesar

doi:10.1117/12.2001708

22 February 2013 Multimodal nonlinear optical microscopy used to discriminate human colon cancer

Javier Adur, Vitor B. Pelegati, Mariana Bianchi, André A. de Thomaz, Mariana O. Baratti, Hernandes F. Carvalho, Víctor H. Casco, Carlos L. Cesar

Author Affiliations +

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881J (2013) https://doi.org/10.1117/12.2001708
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Colon cancer is one of the most diffused cancers in the Western World, ranking third worldwide in frequency of incidence after lung and breast cancers. Even if it is curable when detected and treated early, a more accurate premature diagnosis would be a suitable aim for both cancer prognostic and treatment. Combined multimodal nonlinear optical (NLO) microscopies, such as two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG), third harmonic generation (THG), and fluorescence lifetime imaging microscopy (FLIM) can be used to detect morphological and metabolic changes associated with stroma and epithelial transformation in colon cancer disease. NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns between normal and malignant human colonic mucosa. Using a set of scoring methods significant differences both in the content, distribution and organization of stroma collagen fibrils, and lifetime components of NADH and FAD cofactors of human colon mucosa biopsies were found. Our results provide a framework for using NLO techniques as a clinical diagnostic tool for human colon cancer, and also suggest that the SHG and FLIM metrics could be applied to other intestinal disorders, which are characterized by abnormal cell proliferation and collagen assembly.

Citation Download Citation

Javier Adur, Vitor B. Pelegati, Mariana Bianchi, André A. de Thomaz, Mariana O. Baratti, Hernandes F. Carvalho, Víctor H. Casco, and Carlos L. Cesar "Multimodal nonlinear optical microscopy used to discriminate human colon cancer", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881J (22 February 2013); https://doi.org/10.1117/12.2001708

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available