Chemical-contrast imaging with pulse-shaping based pump-probe spectroscopy

Daniel C. Flynn; Amar R. Bhagwat; Jennifer P. Ogilvie

doi:10.1117/12.2003218

22 February 2013 Chemical-contrast imaging with pulse-shaping based pump-probe spectroscopy

Daniel C. Flynn, Amar R. Bhagwat, Jennifer P. Ogilvie

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881Z (2013) https://doi.org/10.1117/12.2003218
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Ultrafast pump-probe spectroscopy and pulse-shaping techniques are providing new modes of contrast for the field of multiphoton microscopy. Endogenous species such as heme proteins show rich nonlinear spectroscopic signatures of excited state absorption, stimulated emission and ground-state bleaching. Commercially available octave-spanning Ti:sapphire oscillators offer new opportunities for imaging based on pump-probe contrast. Spatial light modulators take advantage of this large bandwidth, shaping pulses of light to selectively excite molecular structures with similar spectral properties. We present two-color pump-probe imaging of heme proteins solutions and red blood cells.

Citation Download Citation

Daniel C. Flynn, Amar R. Bhagwat, and Jennifer P. Ogilvie "Chemical-contrast imaging with pulse-shaping based pump-probe spectroscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881Z (22 February 2013); https://doi.org/10.1117/12.2003218

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