Exploring the physics of single molecules of charged macromolecules by fluorescence correlation spectroscopy

Shuangjiang Luo; Qingbo Yang; Pengxiang Jia; Shengqin Wang; Jingfa Yang; Jiang Zhao

doi:10.1117/12.2007679

22 February 2013 Exploring the physics of single molecules of charged macromolecules by fluorescence correlation spectroscopy

Shuangjiang Luo, Qingbo Yang, Pengxiang Jia, Shengqin Wang, Jingfa Yang, Jiang Zhao

Author Affiliations +

Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI; 859004 (2013) https://doi.org/10.1117/12.2007679
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The high sensitivity at single molecule level of fluorescence correlation spectroscopy (FCS) enables measurements of charged polymer molecules at extreme dilution condition, which has been difficulty for conventional techniques as dynamic light scattering. By FCS, the conformation transition of single chain of polyelectrolytes is clearly exposed: the first-order conformation transition by pH of poly 2-vinylpyridine, the scaling analysis of polystyrene sulfonate shows a change from rod-like conformation to random coil conformation with the increase of the molecular weight. Photon count histogram (PCH) helps to determine the electric potential of single charged polymer molecules, explaining the important contribution of the counterion distribution: the breakdown of “counterion condensation.” FCS is a very important method for the study of charged polymers and bio-macromolecules.

Citation Download Citation

Shuangjiang Luo, Qingbo Yang, Pengxiang Jia, Shengqin Wang, Jingfa Yang, and Jiang Zhao "Exploring the physics of single molecules of charged macromolecules by fluorescence correlation spectroscopy", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859004 (22 February 2013); https://doi.org/10.1117/12.2007679

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