DNA-templated nanoantennas for single-molecule detection at elevated concentrations

G. P. Acuna; P. Holzmeister; F. M. Möller; S. Beater; B. Lalkens; P. Tinnefeld

doi:10.1117/12.2003565

22 February 2013 DNA-templated nanoantennas for single-molecule detection at elevated concentrations

G. P. Acuna, P. Holzmeister, F. M. Möller, S. Beater, B. Lalkens, P. Tinnefeld

Proceedings Volume 8595, Colloidal Nanocrystals for Biomedical Applications VIII; 859509 (2013) https://doi.org/10.1117/12.2003565
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The dynamic concentration range is one of the major limitations of single-molecule fluorescence techniques. Here, we show how bottom-up nano-antennas enhance the fluorescence intensity in a reduced hot-spot, ready for biological applications. We use self-assembled DNA origami structures as a breadboard where gold nanoparticle dimers are positioned with nanometer precision. A maximum of almost 100fold intensity enhancement is obtained using 100 nm gold nanoparticles within a gap of 23 nm between the particles. The results obtained are in good agreement with numerical simulations. Due to the intensity enhancement introduced by the nano-antenna, we are able to perform single molecule measurements at concentrations as high as 500 nM which represents an increment of 2 orders of magnitude compared to conventional measurements. The combination of metallic nanoparticles with DNA origami structures with docking points for biological assays paves the way for the development of bottom-up inexpensive enhancement chambers for single molecule measurements at high concentrations where processes like DNA sequencing occur.

Citation Download Citation

G. P. Acuna, P. Holzmeister, F. M. Möller, S. Beater, B. Lalkens, and P. Tinnefeld "DNA-templated nanoantennas for single-molecule detection at elevated concentrations", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 859509 (22 February 2013); https://doi.org/10.1117/12.2003565

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KEYWORDS

Luminescence

Neptunium

Nanoparticles

Gold

Quantum efficiency

Numerical simulations

Confocal microscopy

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