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Proceedings Article

Supercritical self-interference fluorescence microscopy for full-field membrane imaging

[+] Author Affiliations
Thomas Barroca, Pierre Bon, Emmanuel Fort

Institut Langevin, CNRS, ESPCI ParisTech (France)

Sandrine Lévêque-Fort

Institut des Sciences Moléculaires d'Orsay and Ctr. de photonique Biomédicale, CNRS, Univ. Paris-Sud (France)

Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858911 (February 22, 2013); doi:10.1117/12.2003736
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From Conference Volume 8589

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
  • Carol J. Cogswell; Thomas G. Brown; Jose-Angel Conchello; Tony Wilson
  • San Francisco, California, USA | February 02, 2013

abstract

We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Thomas Barroca ; Pierre Bon ; Sandrine Lévêque-Fort and Emmanuel Fort
" Supercritical self-interference fluorescence microscopy for full-field membrane imaging ", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858911 (February 22, 2013); doi:10.1117/12.2003736; http://dx.doi.org/10.1117/12.2003736


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