We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in
order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering
technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the
microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope
to simultaneously monitor in real time both the cell membrane and in-depth phenomena.
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Citation
Thomas Barroca ; Pierre Bon ; Sandrine Lévêque-Fort and Emmanuel Fort
"
Supercritical self-interference fluorescence microscopy for full-field membrane imaging
", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858911 (February 22, 2013); doi:10.1117/12.2003736; http://dx.doi.org/10.1117/12.2003736
