Supercritical self-interference fluorescence microscopy for full-field membrane imaging

Thomas Barroca; Pierre Bon; Sandrine Lévêque-Fort; Emmanuel Fort

doi:10.1117/12.2003736

22 February 2013 Supercritical self-interference fluorescence microscopy for full-field membrane imaging

Thomas Barroca, Pierre Bon, Sandrine Lévêque-Fort, Emmanuel Fort

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 858911 (2013) https://doi.org/10.1117/12.2003736
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena.

Citation Download Citation

Thomas Barroca, Pierre Bon, Sandrine Lévêque-Fort, and Emmanuel Fort "Supercritical self-interference fluorescence microscopy for full-field membrane imaging", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858911 (22 February 2013); https://doi.org/10.1117/12.2003736

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