Iron oxide nanoparticle (IONP) hyperthermia is an emerging treatment that shows great potential as a cancer
therapy both alone and in synergy with conventional modalities. Pre-clinical studies are attempting to elucidate the
mechanisms of action and distributions of IONP in various in vitro and in vivo models, however these studies would
greatly benefit from real-time imaging of IONP locations both in cellular and in mammalian systems. To this end,
fluorescently-tagged IONP (fIONP) have been employed for real time tracking and co-registration of IONP with iron
content. Starch-coated IONP were fluorescently-tagged, purified and analyzed for fluorescent signal at various
concentrations. fIONP were incubated with MTGB cells for varying times and cellular uptake analyzed using confocal
microscopy, flow cytometry and inductively-coupled plasma mass spectrometry (ICP-MS). fIONP were also injected
into a bilateral mouse tumor model for radiation modification of tumor tissue and enhanced fIONP deposition assessed
using a Xenogen IVIS fluorescent imager. Results demonstrated that fIONP concentrations in vitro correlated with ICPMS
iron readings. fIONP could be tracked in vitro as well as in tissue samples from an in vivo model. Future work will
employ whole animal fluorescent imaging to track the biodistribution of fIONP over time.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jennifer A. Tate ; Mark D. Savellano and P. Jack Hoopes
Biodistribution and imaging of fluorescently-tagged iron oxide nanoparticles in a breast cancer mouse model
", Proc. SPIE 8584, Energy-based Treatment of Tissue and Assessment VII, 85840I (February 26, 2013); doi:10.1117/12.2007607; http://dx.doi.org/10.1117/12.2007607