Measurement of dynamic scattering beneath stationary layers using multiple-exposure laser speckle contrast analysis

Evan Hirst; Oliver Thompson; Mike Andrews

doi:10.1117/12.2005266

1 March 2013 Measurement of dynamic scattering beneath stationary layers using multiple-exposure laser speckle contrast analysis

Evan Hirst, Oliver Thompson, Mike Andrews

Author Affiliations +

Proceedings Volume 8580, Dynamics and Fluctuations in Biomedical Photonics X; 858007 (2013) https://doi.org/10.1117/12.2005266
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The retina/choroid structure is an example of a complex biological target featuring highly perfused tissues and vessel flows both near the surface and at some depth. Laser speckle imaging can be used to image blood flows but static scattering paths present a problem for extracting quantifiable data. The speckle contrast is artificially increased by any residual specular reflection and light paths where no moving scatterers are encountered. Here we present results from phantom experiments demonstrating that the static and dynamic contributions to laser speckle contrast can be separated when camera exposures of varying duration are used. The stationary contrast parameter follows the thickness and strength of the overlying scatterer while the dynamic proportion of the scatter resulting from vessel flows and Brownian motion is unchanged. The importance of separating the two scatter components is illustrated by in vivo measurements from a scarred human retina, where the effect of the un-perfused scar tissue can be decoupled from the dynamic speckle from the intact tissue beneath it.

Citation Download Citation

Evan Hirst, Oliver Thompson, and Mike Andrews "Measurement of dynamic scattering beneath stationary layers using multiple-exposure laser speckle contrast analysis", Proc. SPIE 8580, Dynamics and Fluctuations in Biomedical Photonics X, 858007 (1 March 2013); https://doi.org/10.1117/12.2005266

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