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Previously we demonstrated the utility of optical fluorometry to evaluate lung tissue mitochondrial redox state
in isolated perfused rats lungs under various chemically-induced respiratory states. The objective of this study
was to evaluate the effect of acute ischemia on lung tissue mitochondrial redox state in vivo using optical
fluorometry. Under ischemic conditions, insufficient oxygen supply to the mitochondrial chain should reduce
the mitochondrial redox state calculated from the ratio of the auto-fluorescent mitochondrial metabolic
coenzymes NADH (Nicotinamide Adenine Dinucleotide) and FAD (Flavoprotein Adenine Dinucleotide). The
chest of anesthetized, and mechanically ventilated Sprague-Dawley rat was opened to induce acute ischemia by
clamping the left hilum to block both blood flow and ventilation to one lung for approximately 10 minutes.
NADH and FAD fluorescent signals were recorded continuously in a dark room via a fluorometer probe placed
on the pleural surface of the left lung. Acute ischemia caused a decrease in FAD and an increase in NADH,
which resulted in an increase in the mitochondrial redox ratio (RR=NADH/FAD). Restoration of blood flow
and ventilation by unclamping the left hilum returned the RR back to its baseline. These results (increase in RR
under ischemia) show promise for the fluorometer to be used in a clinical setting for evaluating the effect of
pulmonary ischemia-reperfusion on lung tissue mitochondrial redox state in real time.
R. Sepehr,K. Staniszewski,E. R. Jacobs,S. Audi, andMahsa Ranji
"Fluorometry of ischemia reperfusion injury in rat lungs in vivo", Proc. SPIE 8580, Dynamics and Fluctuations in Biomedical Photonics X, 85800W (1 March 2013); https://doi.org/10.1117/12.2002094
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R. Sepehr, K. Staniszewski, E. R. Jacobs, S. Audi, Mahsa Ranji, "Fluorometry of ischemia reperfusion injury in rat lungs in vivo," Proc. SPIE 8580, Dynamics and Fluctuations in Biomedical Photonics X, 85800W (1 March 2013); https://doi.org/10.1117/12.2002094