Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo

Xin Cai; Yu Zhang; Li Li; Sung-Wook Choi; Matthew R. MacEwan; Junjie Yao; Chulhong Kim; Younan Xia; Lihong V. Wang

doi:10.1117/12.2005236

4 March 2013 Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo

Xin Cai, Yu Zhang, Li Li, Sung-Wook Choi, Matthew R. MacEwan, Junjie Yao, Chulhong Kim, Younan Xia, Lihong V. Wang

Proceedings Volume 8581, Photons Plus Ultrasound: Imaging and Sensing 2013; 858128 (2013) https://doi.org/10.1117/12.2005236
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

It is a challenge to non-invasively visualize in vivo the neovascularization in a three-dimensional (3D) scaffold with high spatial resolution and deep penetration depth. Here we used photoacoustic microscopy (PAM) to chronically monitor neovascularization in an inverse opal scaffold implanted in a mouse model for up to six weeks. The neovasculature was observed to develop gradually in the same mouse. These blood vessels not only grew on top of the implanted scaffold but also penetrated into the scaffold. The PAM system offered a lateral resolution of ~45 μm and a penetration depth of ~3 mm into the scaffold/tissue construct. By using the 3D PAM data, we further quantified the vessel area as a function of time.

Citation Download Citation

Xin Cai, Yu Zhang, Li Li, Sung-Wook Choi, Matthew R. MacEwan, Junjie Yao, Chulhong Kim, Younan Xia, and Lihong V. Wang "Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858128 (4 March 2013); https://doi.org/10.1117/12.2005236

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

3D image processing

Photoacoustic microscopy

3D photoacoustic microscopy

Blood

In vivo imaging

Blood vessels

Ear

Show All Keywords

Keywords/Phrases

Search In:

Publication Years