Viewing individual cells and ambient microvasculature using two molecular contrasts

Zhixing Xie; Sung-Liang Chen; Mario L. Fabiilli; J. Brian Fowlkes; K. Kirk Shung; Qifa Zhou; Xunbin Wei; Paul L. Carson; Xueding Wang

doi:10.1117/12.2004666

4 March 2013 Viewing individual cells and ambient microvasculature using two molecular contrasts

Zhixing Xie, Sung-Liang Chen, Mario L. Fabiilli, J. Brian Fowlkes, K. Kirk Shung, Qifa Zhou, Xunbin Wei, Paul L. Carson, Xueding Wang

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Proceedings Volume 8581, Photons Plus Ultrasound: Imaging and Sensing 2013; 85813G (2013) https://doi.org/10.1117/12.2004666
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

To view the individual cells and ambient microvasculature simultaneously will be helpful to study tumor angiogenesis and microenvironments. To achieve this, two molecular contrast mechanisms were exploited simultaneously by integrating two imaging modalities, confocal fluorescence microscopy (CFM) and photoacoustic microscopy (PAM). These share the same scanning optical path and laser source. The induced photoacoustic (PA) signal was detected by a highly sensitive needle hydrophone; while the back-traveling fluorescent photons emitted from the same sample were collected by an avalanche photodetector. Experiments on ex vivo rat bladders were conducted. The CFM image depicted the shape and size of the individual cells successfully. Besides large polygonal umbrella cells, some intracellular components can also be discerned. With the CFM image presenting morphologic cellular information in the bladder wall, the PAM image provides the complementary information, based on the endogenous optical absorption contrast, of the microvascular distribution inside the bladder wall, from large vessels to capillaries. Such multimodal imaging provides the opportunity to realize both histological assay and characterization of microvasculature using one imaging setup. This approach offers the possibility of comprehensive diagnosis of cancer in vivo.

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Zhixing Xie, Sung-Liang Chen, Mario L. Fabiilli, J. Brian Fowlkes, K. Kirk Shung, Qifa Zhou, Xunbin Wei, Paul L. Carson, and Xueding Wang "Viewing individual cells and ambient microvasculature using two molecular contrasts", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85813G (4 March 2013); https://doi.org/10.1117/12.2004666

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KEYWORDS

Bladder

Luminescence

Imaging systems

Confocal microscopy

Signal detection

In vivo imaging

Photoacoustic spectroscopy

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