In vivo detection of circulating tumor cells during tumor manipulation

Mazen A. Juratli; Ekaterina I. Galanzha M.D.; Mustafa Sarimollaoglu; Dmitry A. Nedosekin; James Y. Suen M.D.; Vladimir P. Zharov

doi:10.1117/12.2007986

8 March 2013 In vivo detection of circulating tumor cells during tumor manipulation

Mazen A. Juratli, Ekaterina I. Galanzha M.D., Mustafa Sarimollaoglu, Dmitry A. Nedosekin, James Y. Suen M.D., Vladimir P. Zharov

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Proceedings Volume 8565, Photonic Therapeutics and Diagnostics IX; 85652H (2013) https://doi.org/10.1117/12.2007986
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Melanoma of the head and neck and its treatment are complex issues. The behavior of head and neck melanoma is aggressive, and it has an overall poorer prognosis than of other skin sites. The goal of this research was to determine if melanoma manipulation could enhance penetration of cancer cells from the primary tumor into the circulatory system. Nude mice were inoculated with melanoma cells in the mouse ear. Blood vessels were monitored for the presence of circulating tumor cells (CTCs) using in vivo photoacoustic (PA) flow cytometry (PAFC). The implanted tumor underwent compression, incisional biopsy, or surgical excision, and the release of CTCs was monitored using in vivo PAFC in real time. We discovered that some medical procedures, like compression of an implanted tumor of a mouse model or an incisional biopsy, may either initiate CTC release in the blood which previously contained no CTCs or dramatically increased (10-30–fold) CTC counts above the initially recorded level. These findings indicate that some intervention (such as palpation during physical exam, or incisional biopsy) can potentially enhance penetration of cancer cells from a primary tumor into the blood circulation, which may increase the risk of metastases. Our results warn oncologists of at least some precaution during physical examination, careful surgery strategy.

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Mazen A. Juratli, Ekaterina I. Galanzha M.D., Mustafa Sarimollaoglu, Dmitry A. Nedosekin, James Y. Suen M.D., and Vladimir P. Zharov "In vivo detection of circulating tumor cells during tumor manipulation", Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85652H (8 March 2013); https://doi.org/10.1117/12.2007986

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