Orthogonal diffuse near-infrared reflectance spectroscopy allows to assess cerebral dysfunction and temperature variations following heatstroke on a mouse model

David Abookasis; Elad Zafrir; Elimelech Nesher; Albert Pinhasov; Shmuel Sternklar; Marlon S. Mathews

doi:10.1117/12.2005826

8 March 2013 Orthogonal diffuse near-infrared reflectance spectroscopy allows to assess cerebral dysfunction and temperature variations following heatstroke on a mouse model

David Abookasis, Elad Zafrir, Elimelech Nesher, Albert Pinhasov, Shmuel Sternklar, Marlon S. Mathews

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Proceedings Volume 8565, Photonic Therapeutics and Diagnostics IX; 85654N (2013) https://doi.org/10.1117/12.2005826
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this study, we demonstrate the use of orthogonal diffuse reflectance spectroscopy (o-DRS) to assess brain dysfunction and to monitor internal temperature variations during heatstroke in intact mice brains (n=6). Heatstroke is a medical emergency defined by abnormally elevated body temperature greater than 40°C that causes biochemical, physiological and hematological changes (multiorgan damage). Therefore, quick diagnosis and management of heatstroke victims is essential for good outcomes. Current clinical methods for monitoring temperature (invasive and noninvasive) suffers from several drawbacks such as complexity, cost, portability, safety, etc. To overcomes these deficiencies, a DRS working at the spectral range of 600-1000nm in orthogonal mode together with numerical processing have been applied to First, monitor cerebral optical changes, Second, evaluate rise in temperature and Third, to predict internal temperature noninvasively. Heatstroke was induced by exposing of the anesthetized mouse body, placed above controlled heating pad, to a high ambient temperature with increasing intervals of 1°C until death. Experimental results show variations in both absorption and scattering during heatstroke which emphasizes the changes in brain chromophores and morphology that occur during temperature elevation. In addition, a reflectance-temperature index was developed and found to correlate well with the measured temperature. Our preliminary results suggest that our o-DRS have the potential to monitor and assess internal temperature variations and thus may serve as a useful tool in clinical and laboratory settings.

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David Abookasis, Elad Zafrir, Elimelech Nesher, Albert Pinhasov, Shmuel Sternklar, and Marlon S. Mathews "Orthogonal diffuse near-infrared reflectance spectroscopy allows to assess cerebral dysfunction and temperature variations following heatstroke on a mouse model", Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85654N (8 March 2013); https://doi.org/10.1117/12.2005826

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KEYWORDS

Temperature metrology

Brain

Scattering

Absorption

Absorbance

Diffuse reflectance spectroscopy

Optical properties

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