Laser speckle contrast reveals cerebral blood flow dynamics evoked by
optogenetically controlled neuronal activity

Nan Li; Nitish V. Thakor; Galit Pelled

doi:10.1117/12.2009830

8 March 2013 Laser speckle contrast reveals cerebral blood flow dynamics evoked by optogenetically controlled neuronal activity

Nan Li, Nitish V. Thakor, Galit Pelled

Author Affiliations +

Proceedings Volume 8586, Optogenetics: Optical Methods for Cellular Control; 85860F (2013) https://doi.org/10.1117/12.2009830
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

As a critical basis of functional brain imaging, neurovascular coupling describes the link between neuronal and hemodynamic changes. The majority of in vivo neurovascular coupling studies was performed by inducing sensory stimulation via afferent inputs. Unfortunately such an approach results in recruiting of multiple types of cells, which confounds the explanation of neuronal roles in stimulus evoked hemodynamic changes. Recently optogenetics has emerged to provide immediate control of neurons by exciting or inhibiting genetically engineered neurons expressing light sensitive proteins. However, there is a need for optical methods capable of imaging the concurrent hemodynamic changes. We utilize laser speckle contrast imaging (LSCI) to obtain high resolution display of cerebral blood flow (CBF) in the vicinity of the targeted neural population. LSCI is a minimally invasive method for imaging CBF in microvessels through thinned skull, and produces images with high spatiotemporal resolution, wide field of view. In the integrated system light sources with different wavelengths and band-passing/blocking filters were used to allow simultaneous optical manipulation of neuronal activities and optical imaging of corresponding CBF. Experimental studies were carried out in a rodent model expressing channalrhodopsin (ChR2) in excitatory neurons in the somatosensory cortex (S1). The results demonstrated significant increases of CBF in response to ChR2 stimulation (exciting neuronal firing) comparable to the CBF response to contralateral forepaw stimulation. The approach promises to be an exciting minimally invasive method to study neurovascular coupling. The complete system provides a novel approach for broad neuroscience applications.

Citation Download Citation

Nan Li, Nitish V. Thakor, and Galit Pelled "Laser speckle contrast reveals cerebral blood flow dynamics evoked by optogenetically controlled neuronal activity", Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860F (8 March 2013); https://doi.org/10.1117/12.2009830

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available