Recently developed optogenetics techniques have enabled researchers to modulate the activity of specific cell types. As a
result, complex neural pathways previously regarded as black boxes can now be directly probed, yielding a steadily
increasing understanding of the basic neural circuits that underlie health and disease.
For in vivo experimentation, fiber-coupled lasers have traditionally been used to illuminate internal brain regions, via an
optical fiber that penetrates through overlying tissue. Though able to deliver intense fiber-coupled light, lasers are costly,
bulky, and face limitations in output beam stability and temporal precision during modulated outputs. For experiments
on unrestricted, behaving animals, a laser-based system also necessitates the use of fiber optic rotary joints, which come
with costs and limitations of their own.
Here, we report and characterize an alternative light delivery solution, based on high intensity fiber-coupled LEDs that
are miniaturized for placement on the end of custom electrical commutators. This design allows for enhanced control of
output light and expanded capabilities for optical stimulation as well as simultaneous electrical neural recordings, as with
an optrode array.
Temporal response of light outputs and light stability during commutator rotation were assessed. The influence of high
current optical control signals on adjacent neural recording channels was also explored. To validate the function of this
LED based system in in vivo recording scenarios, chronic stimulation experiments were performed.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Isaac P. Clements ; Andrew G. Gnade ; Alexander D. Rush ; Craig D. Patten ; Mark C. Twomey, et al.
Miniaturized LED sources for in vivo optogenetic experimentation
", Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860X (March 8, 2013); doi:10.1117/12.2008080; http://dx.doi.org/10.1117/12.2008080