Photodynamic Therapy (PDT) has proven to be an effective treatment option for nonmelanoma skin cancers. The ability
to quantify the concentration of drug in the treated area is crucial for effective treatment planning as well as predicting
outcomes. We utilized spatial frequency domain imaging for quantifying the accurate concentration of protoporphyrin IX
(PpIX) in phantoms and in vivo. We correct fluorescence against the effects of native tissue absorption and scattering
parameters. First we quantified the absorption and scattering of the tissue non-invasively. Then, we corrected raw
fluorescence signal by compensating for optical properties to get the absolute drug concentration. After phantom
experiments, we used basal cell carcinoma (BCC) model in Gli mice to determine optical properties and drug
concentration in vivo at pre-PDT.
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Citation
Ulas Sunar ; Daniel Rohrbach ; Janet Morgan and Natalie Zeitouni
"
Noninvasive imaging of absolute PpIX concentration distribution in
nonmelanoma skin tumors at pre-PDT
", Proc. SPIE 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII, 85680C (March 13, 2013); doi:10.1117/12.2005078; http://dx.doi.org/10.1117/12.2005078
