In vivo estimation of light scattering and absorption properties of rat brain using single reflectance fiber probe during cortical spreading depression

Izumi Nishidate; Chiharu Mizushima; Keiichiro Yoshida; Satoko Kawauchi; Shunichi Sato; Manabu Sato

doi:10.1117/12.2003049

25 March 2013 In vivo estimation of light scattering and absorption properties of rat brain using single reflectance fiber probe during cortical spreading depression

Izumi Nishidate, Chiharu Mizushima, Keiichiro Yoshida, Satoko Kawauchi, Shunichi Sato, Manabu Sato

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Proceedings Volume 8578, Optical Tomography and Spectroscopy of Tissue X; 85782Y (2013) https://doi.org/10.1117/12.2003049
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Diffuse reflectance spectroscopy using fiber optic probe is one of most promising technique for evaluating optical properties of biological tissue. We present a method determining the reduced scattering coefficients μ_s’, the absorption coefficients μ_a, and tissue oxygen saturation StO₂ of in vivo brain tissue using single reflectance fiber probe with two source-collector geometries. In this study, we performed in vivo recordings of diffuse reflectance spectra and the electrophysiological signals for exposed brain of rats during the cortical spreading depression (CSD) evoked by the topical application of KCl. The time courses of μ_a in the range from 500 to 584 nm and StO₂ indicated the hemodynamic change in cerebral cortex. Time courses of μ_s’ are well correlated with those of μ_a in the range from 530 to 570 nm, which also reflect the scattering by red blood cells. On the other hand, increases in μ_s’ at 500 and 584 nm were observed before the profound increase in μ_a and they synchronized with the negative DC shift of the local field potential. It is said that the DC shift coincident with a rise in extracellular potassium and can evoke cell deformation generated by water movement between intracellular and extracellular compartments, and hence the light scattering by tissue. Therefore, the increase in μ_s’ at 500 and 584 nm before the profound increase in μ_a are indicative of changes in light scattering by tissue. The results in this study indicate potential of the method to evaluate the pathophysiological conditions of in vivo brain.

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Izumi Nishidate, Chiharu Mizushima, Keiichiro Yoshida, Satoko Kawauchi, Shunichi Sato, and Manabu Sato "In vivo estimation of light scattering and absorption properties of rat brain using single reflectance fiber probe during cortical spreading depression", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85782Y (25 March 2013); https://doi.org/10.1117/12.2003049

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KEYWORDS

Absorption

Scattering

Tissue optics

Light scattering

In vivo imaging

Brain

Monte Carlo methods

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