Salivary proteome as an in vivo model to study breast cancer progression

Charles F. Streckfus

doi:10.1117/12.2020016

29 May 2013 Salivary proteome as an in vivo model to study breast cancer progression

Charles F. Streckfus

Proceedings Volume 8723, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III; 87230D (2013) https://doi.org/10.1117/12.2020016
Event: SPIE Defense, Security, and Sensing, 2013, Baltimore, Maryland, United States

Abstract

Objective: The objective of this experiment was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with breast cancer with and without HER2/neu overexpression in an attempt to demonstrate how these profiles may be used to study breast cancer progression. Methods: Pooled (n=10 pooled) stimulated whole saliva specimens from women were analyzed. One pooled specimen was from women diagnosed with Stage IIa (T₂N₀M₀) invasive ductal carcinoma (IDC) without positive HER2/neu receptor status. A second pooled specimen was from women diagnosed with Stage IIa (T₂N₀M₀) IDC with a positive HER2/neu receptor status. Experimentally, saliva from each of the pooled samples was trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase (C18) capillary chromatography on an LC-MS/MS mass spectrometer equipped with an LC-Packings HPLC. Results: The results yielded 34 up-regulated proteins and 37 down regulated proteins that were differentially expressed between HER2/neu receptor positive HER2/neu receptor negative specimens. Conclusions: This pilot study provides support to the novel idea of using salivary secretions to study breast cancer progression.

Citation Download Citation

Charles F. Streckfus "Salivary proteome as an in vivo model to study breast cancer progression", Proc. SPIE 8723, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III, 87230D (29 May 2013); https://doi.org/10.1117/12.2020016

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