The efforts aimed at early diagnosis of oral cancer should be prioritized towards developing a new screening instrument, based on optical coherence tomography (OCT), to be used directly intraorally, able to perform a fast, real time, 3D and non-invasive diagnosis of oral malignancies. The first step in this direction would be to optimize the OCT image interpretation of oral tissues. Therefore we propose plastination as a tissue preparation method that better preserves three-dimensional structure for study by new optical imaging techniques. The OCT and the synchrotron radiation computed microtomography (micro-CT) were employed for tissue sample analyze. For validating the OCT results we used the gold standard diagnostic procedure for any suspicious lesion – histopathology. This is a preliminary study of comparing features provided by OCT and Micro-CT. In the conditions of the present study, OCT proves to be a highly promising imaging modality. The use of x-ray based topographic imaging of small biological samples has been limited by the low intrinsic x-ray absorption of non-mineralized tissue and the lack of established contrast agents. Plastination can be used to enhance optical imagies of oral soft tissue samples.
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Silvana Cânjău ; Carmen Todea ; Cosmin Sinescu ; Meda L. Negruţiu ; Virgil Duma, et al.
Optical imaging of oral pathological tissue using optical coherence tomography and synchrotron radiation computed microtomography
", Proc. SPIE 8805, Head and Neck Optical Diagnostics, 880504 (June 24, 2013); doi:10.1117/12.2032584; http://dx.doi.org/10.1117/12.2032584