The precision in localizing a molecule is ultimately determined by the number of detected photons, which is in turn limited by photobleaching. Currently, fluorophores can be routinely localized to a few tens of nanometers at room temperature. In this work we demonstrate localization precision better than 3 Angstrom by substantial improvement of the molecular photostability at cryogenic temperatures. We discuss the challenges, solutions and promise of our methodology for high-performance co-localization and super-resolution microscopy.
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Siegfried Weisenburger ; Bo Jing ; Alois Renn and Vahid Sandoghdar
Cryogenic localization of single molecules with angstrom precision
", Proc. SPIE 8815, Nanoimaging and Nanospectroscopy, 88150D (September 20, 2013); doi:10.1117/12.2025373; http://dx.doi.org/10.1117/12.2025373