LED uniform illumination system for DMD-based confocal microscopy

Kaimin Xiao; Wenmei Hou; Qixin Xu; Bofang Peng

doi:10.1117/12.2035730

10 October 2013 LED uniform illumination system for DMD-based confocal microscopy

Kaimin Xiao, Wenmei Hou, Qixin Xu, Bofang Peng

Proceedings Volume 8916, Sixth International Symposium on Precision Mechanical Measurements; 89163T (2013) https://doi.org/10.1117/12.2035730
Event: Sixth International Symposium on Precision Mechanical Measurements, 2013, Guiyang, China

Abstract

Due to the coherence of laser light source it could produce coherent noise in parallel confocal microscopy based on Digital Micromirror Device (DMD) and thus affect the resolution. LED light source instead of the laser light source can give full play because of its incoherence characterization. In this paper, free-form surface lens is used for LED secondary optics design. According to the LED characteristics and the law of refraction, we have established differential equations of free-form surface. We solved equations with the method of Runge-Kutta by Matlab and the model was built in Tracepro for optical simulation. The results show that the uniformity on the DMD is better than 90% and the lighting efficiency is higher than before. The measured data show us a much more uniform illumination on DMD and LED uniform illumination system successfully avoided the gray error which was caused by the uneven illumination. The LED driver circuit using DC power supply provides us a more stable light source. The axial optical tomography is more accurate and the reconstruction of three-dimensional image is more clearer.

Citation Download Citation

Kaimin Xiao, Wenmei Hou, Qixin Xu, and Bofang Peng "LED uniform illumination system for DMD-based confocal microscopy", Proc. SPIE 8916, Sixth International Symposium on Precision Mechanical Measurements, 89163T (10 October 2013); https://doi.org/10.1117/12.2035730

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available